Variability in commercial histoplasma complement fixation antigens

Variation in complement fixation test results with three Histoplasma capsulatum yeast phase antigens.

Antigens derived from three different yeast strains of Histoplasma capsulatum (GW and VC used by the Veterans Administration Serology Reference Laboratory and the Centers for Disease Control reference antigen, A811) were compared with each other to determine their equivalency. Results from 200 sera, tested concurrently by the Serology Reference Laboratory and the University of Kentucky Mycosero...

Evaluation of histoplasmin and yeast phase antigens derived from a single strain of Histoplasma capsulatum in the complement fixation test.

A complement fixation procedure for the serological diagnos of histoplasmosis in a human patient was attempted first in 1941 by Van Pernis, Benson, and Hollinger. The results were negative with an antigen which consisted of the undiluted filtrate (histoplasmin) from a glucose broth medium inoculated with Hidtoplasma capsulatum. Since that time other types of antigens have been developed by vari...

Indirect Complement Fixation in Foot-and-Mouth Disease

Complement Fixation in Diseased Tissues

An immunohistologic complement fixation test has been used in an effort to detect immune complexes in sections of kidney from rats injected with rabbit anti-rat kidney serum and in sections of biopsied kidneys from four humans with membranous glomerulonephritis. Sections of the rat and human kidneys were treated with fluorescein-conjugated anti-rabbit globulin or antihuman globulin respectively...

Complement Fixation Tests in Pertussis.

Bordet and Gengou (1), in 1906, performed serological tests on pertussis employing the organisms which they discovered and claimed to be the causative agent of whooping cough as the antigen for the complement fixation test. They got a positive reaction in most cases of the disease. Yet, later investigations failed to confirm these results. Among those who confirmed their results were Renaux (2)...